Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria.
BACKGROUND:PCR in principle can detect a single target molecule in a reaction mixture.Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples.The most pernicious source of contamination is microbial DNA in DNA polymerase preparations.Importantly, al